For total RNA extraction from whole blood & cultured cells

Advantages

• Rapid purification of high-quality and high yields, ready-to-use RNA

• Purify total RNA within 20 minutes!

• No organic extraction or alcohol precipitation

• Removal of contaminants and inhibitors

• Certified RNase -free

Specifications

Components

• RBC Lysis Buffer

• IRL Buffer

• RW1 Buffer

• RW2 Buffer (Add Ethanol)

• Elution Buffer (DEPC H2O)

• IR Column

• 2 ml Collection Tubes

Functional Test Data

Fig 1. Total RNA extracted from Whole Blood
Total RNA from 200 µl  whole blood samples was extracted using the ImaSpin® Total RNA Extraction Kit - Whole Blood & Cell & Competitive brand Q. 10 µl from 60 µl eluates of purified Total RNA was analyzed by electrophoresis on a 1 % agarose gel.

1-2 = ImaSpin®  Total RNA Extraction Kit -Whole Blood & Cell

3-4 = Competitive brand Q

M = 1 Kb DNA Ladder

Fig 2. Total RNA extracted from cultured cells

Total RNA from cultured 5x 10⁶ HeLa Cell samples was extracted using the ImaSpin® Total RNA Extraction Kit - Whole Blood & Cell & Compeititive brand G & Compeititive brand Q. 10 µl from 60 µl eluates of purified Total RNA was analyzed by electrophoresis on a 1 % agarose gel.

M = 1 Kb DNA Ladder

1-2 = ImaSpin®  Total RNA Extraction Kit -Whole Blood & Cell

3-4 = Competitive brand G

5-6 = Competitive brand Q

Fig 3. Cultured cells Total RNA Extraction stability confirmation

Six repetitions of total RNA extraction from cultured 5x 10⁶ HeLa Cell samples using the ImaSpin® Total RNA Extraction Kit - Whole Blood & Cell. 10 µl from 60 µl eluates of purified Total RNA was analyzed by electrophoresis on a 1% agarose gel.

1-6 = ImaSpin®  Total RNA Extraction Kit -Whole Blood & Cell